MD Biosciences offers a diverse selection of well-characterized ischemic stroke models to study injury mechanisms and cell damage. Our focal ischemia (MCAo, tMCAo) models closely replicate human stroke, offering valuable insights into therapeutic interventions, while our global ischemia (4VO) models are particularly relevant for conditions such as cardiac arrest. These mouse and rat stroke models are crucial for evaluating the efficacy of therapies targeting neurodegeneration and neuroinflammation. To provide a comprehensive analysis, additional readouts like behavior, biomarker analysis, and histology, are seamlessly integrated into our studies.
Models for Stroke and Ischemia:
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MCAo - Focal Ischemia
The focal cerebral ischemic model (MCAo) of stroke is a widely used model for assessing ischemic brain injury. By occluding the middle cerebral artery, this model induces a reduction in cerebral blood flow, affecting both the striatum and cortex, and mimicking focal brain damage observed in human stroke. We offer two models, permanent occlusion, which causes extensive damage, and transient occlusion, where the artery is temporarily blocked before reperfusion.
Download the stroke model datasheet.
Thionine Staining of Coronal Sections of Rat Brain Tissue. Figures A & C. Normal Rat Brain Tissue. Figures B. & D. Rat brain 72 hours post-MCAo, demonstrating clear damage due to infarct in the right hemisphere.
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4VO - Global Ischemia
The global ischemia model involves the permanent occlusion of vertebral arteries and the transient occlusion of carotid arteries, leading to brain injury in the hippocampus due to reduced cerebral blood flow. This model mimics conditions such as cardiac arrest and coronary bypass surgery.
The 4VO model is used to evaluate the effects of reduced blood flow to the brain, as seen in cardiac arrest and coronary bypass surgery. It is also used to assess neurodegeneration, dementia, and oxidative stress.
Download the stroke model datasheet.
Water Maze Test. Rats that received the test item were able to find the platform in the water maze test in a similar fashion to sham animals. The vehicle treated animals were unable to find the platform after 8 trials.
Scientific Data
Laser Doppler Flowmetry (LDF) data can be collected to monitor cerebral blood flow (CBF) during surgery. Figure: Blood flow was recorded with LDF at baseline and at occlusion commencement. Blood flow reduces at occlusion and increases to baseline levels after reperfusion, indicating that the surgery was successful.
Ischemia Models Datasheet
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