Rapid In vitro screening for degeneration
These in vitro screening assays utilize primary neurons from immature mice or rats. Following the conditioning phase, cultures are treated with compounds and evaluated for their neurodegeneration index. The assays are ideal for screening compounds prior to efficacy in in vivo studies.
A hallmark of degeneration is the blebbing of neurites, which is when neurites begin disassembly and degeneration. The neurodegeneration index (NI) compares the relative area of neurites and the number of blebs.
Neurodegeneration Screening Assays
-
CIPN Screening Assay
Dorsal root ganglion (DRG) neurons were cultured from adult rats. Cultures are treated for 5 days with a chemotherapeutic agent followed by treatment with compound. Cultures are stained with beta-tubulin III antibody and measured for neurodegeneration index.
-
Diabetes Screening Assay
Cortical neurons from immature rat or mice were cultured and conditioned with high concentrations of glucose. After 5 days, the cultures were treated with test item, stained with beta-tubulin II antibody and evaluated for the neurodegeneration index.
-
Alzheimer's Screening Assay
Cortical neurons from immature mice or rats are cultured and conditioned with beta-amyloid for 5 days. Cultures are treated with compound, stained with Beta-tubulin III antibody.
-
Parkinson's Screening Assay
Cortical neurons from immature rats or mice are conditioned for 5 days with a-synuclein. Cultures are treated with compound and stained with Beta-tubulin III antibody staining.
Scientific Data
Staining shows Neurodegeneration following exposure to chemotherapeutic agent, observed by the reduction in neurite area.
After 5 days of conditioning, there is an appearance of blebs. When the test item is added to the culture, the blebs decrease.
Review the complete dataset.
Neurodegeneration Screening Assay Datasheet
Complete the short form for instant access to the complete data set.