Peripheral Nerve Injury 

In the SNL model of peripheral neuropathy, the L5 and L6 spinal nerves are isolated and can either be cut or tightly ligated with surgical silk. Axonal degeneration occurs, with all types of axons being approximately equally affected. Pronounced mechanical allodynia follows, accompanied by spontaneous pain behaviors, cold allodynia, which lasts for months with no recovery. 

View the Spinal Nerve Ligation Model

In our enhanced model, the left sciatic nerve is exposed in anesthetized animals above the femoral joint. One loose silk suture knot and 3 cat gut sutures are applied to the sciatic nerve. The skin is then clamped closed. One week later, the animals' pain threshold is assessed using the von Frey filament test. Animals that demonstrate reduced threshold in the operated leg are included in the study and divided into different treatment groups. The pain threshold is then re-assessed following compound dosing. 

Learn more about the Chronic Constriction Injury model

Neuropathic pain may arise from many different disease states and present with a variety of symptoms, including shooting or burning pain, tingling, numbness, and allodynia, Clinically significant relief is often difficult to achieve, in part because conventional opioid therapy is typically less effective for neuropathic pain. Understanding the mechanisms and underlying neuropathic pain syndromes is crucial to the development of more effective therapies.

Click here for the sciatic nerve crush model

The mouse nerve cuff model is based on a compression of the sciatic nerve using a polyethylene cuff that is implanted around the main branch of the sciatic nerve. This model produces more consistent pain with less variability than other mouse models of peripheral nerve injury (PNI).  

Click here for the sciatic nerve cuff model. 

Models of nerve injury are useful in evaluating compound effects on nerve regeneration. The stretch model is a model of nerve injury that involves a later onset of functional recovery compared to the crush model. 

Click here for the stretch-induced nerve injury model. 

The Spared Nerve Injury (SNI) model is a partial denervation model, in which the common peroneal and tibial nerves are injured, producing consistent and reproducible tactile hypersensitivity in the skin territory of the spared, intact sural nerve. 

Click here for the spared nerve injury (SNI) model.

In the distal tibial injury model, the tibial nerve is dissected and severed as close to its distal end as possible, ensuring avoidance of the popliteal vessels. This model directly reflects this clinical reality, ensuring its relevance and practicality in real-world scenarios.

Click here for the distal tibial injury model.

Preclinical rodent models largely contribute to our understanding of chronic neuropathic pain, however these animal models are limiting due to poor clinical translation. Since pigs share similarities with humans, such as skin innervation and neurological resemblance, MD Biosciences Neuro developed a pig model for chronic pain caused by surgically-induced peripheral neuritis (PNT) (Castel et al., 2016). In the model, 75% of animals exhibit mechanical and tactile allodynia by day 28 post surgery, demonstrating painful neuropathy. We also showed that positive controls inhibited hypersensitivity to von Frey filaments and feather stimuli, reversing spontaneous pain-related behavior in a dose-related manner. 

Click here for more information on the translational pig model of PNT