Spinal Cord Injury
Evaluating neuro-regenerative and neuroprotective therapies.
Overview
Spinal cord injury (SCI) often results in sensory problems, severe motor dysfunction (such as complete paralysis) and chronic pain, making spinal cord injury research complex and challenging. Several preclinical models are commonly used to evaluate pain and motor dysfunction mechanisms associated with SCI.
Spinal Cord Injury Models
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Spinal Cord Injury in vitro assay
Spinal cord injury in a dish uses primary cells dissociated directly from adult (>5 weeks old) rat spinal cord tissues, plated in media and incubated with test items or controls.
Readouts:
- Synaptic imaging for synapse formation
- Neurite outgrowth
- Monitor cells longitutidally
- Immunostaining for cytoskeletal antigens (tubulin III)
Staining with anti-synaptobrevin2
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Spinal Cord Injury in rats
Spinal cord injury in rats created by contusion, cutting, or extraction.
Readouts:
- Basso, Beattie and Bresnahan (BBB) score
- Open field assay
- Walking gait (catwalk)
- Electrophysiology
- Spinal cord histology
Assessments
MD Biosciences incorporates a range of in vivo measures and endpoint assessments that provide a robust data package that enable researchers to make critical decisions.
Behavior
Pain behavior tests assess pain and motor dysfunction.
Biomarkers
Evaluate biomarkers from spinal cord samples.
Histology
Histological staining to damage in the spinal cord.
Electrophysiology
Evaluate MEP and SEP signal data using in vivo electrophysiology.
Scientific Data
H&E staining of the spinal cord 13 weeks post SCI in female rat with a Basso, Beattie, and Bresnahan score (BBB) score of 12.
Review the complete dataset.
Spinal Cord Injury Datasheet
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