Standard Chronic Constriction Injury (CCI)
In the standard CCI model, the left sciatic nerve is ligated causing inflammation, swelling, and ultimately, peripheral pain. This method results in a severe and disproportional reduction of large myelinated fibers. Although significant disruption of small unmyelinated C-fibers occurs as well, many remain intact. For this reason, pain behaviors and increased sensitivity to stimuli is observed following surgery.
Enhanced CCI model
In our enhanced model, the left sciatic nerve is exposed in anesthetized animals above the femoral joint. One loose silk suture knot and 3 cat gut sutures are applied to the sciatic nerve. The skin is then clamped closed. One week later, the animals' pain threshold is assessed using the von Frey filament test. Animals that demonstrate reduced threshold in the operated leg are included in the study and divided into different treatment groups. The pain threshold is then re-assessed following compound dosing.
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