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Sciatic Nerve Cuff

Peripheral Nerve Injury (PNI) Models of Neuropathic Pain

Sciatic Nerve Cuff Model of Peripheral Neuropathic Pain

 

The mouse nerve cuff model is based on a compression of the sciatic nerve using a polyethylene cuff that is implanted around the main branch of the sciatic nerve. This model produces more consistent pain with less variability than other mouse models of peripheral nerve injury (PNI).  

 

Model Specifications

  • Model length: up to 30 days
  • Readouts: tactile allodynia (von Frey), cold allodynia
  • Endpoints: histology, biomarker analysis (protein or mRNA levels in sciatic nerve or serum/plasma)

Assessments

Neurological disorders often result in a combination of motor and cognitive deficits. Thus, behavioral assessments in conjunction with physiological readouts offer a broader understanding of the basic biological mechanism of disease and cognitive impairment, highly relevant to therapeutic developments. 
Pain behavioral anaysis

Behavior 

Pain behavior tests assess the response to various pain stimuli.  
biomarker

Biomarkers

Evaluate pro-inflammatory and anti-inflammatory biomarkers from CSF samples. 
histology

Histology

IHC and histological staining to evaluate inflammatory markers in the paw skin. 
electrophysiology

Electrophysiology

Evaluate spontaneous nerve firing using in vivo electrophysiology.

Scientific Data

Pain response using Von Frey in the nerve cuff model. 

 
mouse_cuff_VF
 
 
Review the complete dataset.

Nerve injury datasheet (1)

Download datasheet
 

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If you are ready to discuss how a partnership can fit into your development program, our scientists are eager to explore the possibilities with you. Like many other pharmaceutical and medical device developers, you can rely on predictive preclinical data.

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