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Spinal Nerve Ligation (SNL)

A preclinical peripheral nerve injury model for neuropathic pain. 

 

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Preclinical Neuropathic Pain Model

 

In the SNL model of peripheral neuropathy, the L5 and L6 spinal nerves are isolated and can either be cut or tightly ligated with surgical silk. Axonal degeneration occurs, with all types of axons being approximately equally affected. Pronounced mechanical allodynia follows, accompanied by spontaneous pain behaviors, cold allodynia, which lasts for months with no recovery. 



Study Options

  • Rat L5/L6 ligation
  • Rat L5 only ligation
  • Model length: up to 30 days
  • Readouts: tactile allodynia (von Frey)
  • Endpoints: histology, biomarker analysis (protein or mRNA levels in sciatic nerve or serum/plasma)
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Assessments

Neurological disorders often result in a combination of motor and cognitive deficits. Thus, behavioral assessments in conjunction with physiological readouts offer a broader understanding of the basic biological mechanism of disease and cognitive impairment, highly relevant to therapeutic developments. 

 
Pain behavioral anaysis

Behavior 

Pain behavior tests assess the response to various pain stimuli.  
biomarker

Biomarkers

Evaluate pro-inflammatory and anti-inflammatory biomarkers from CSF samples. 
histology

Histology

IHC and histological staining to evaluate inflammatory markers in the paw skin. 
electrophysiology

Electrophysiology

Evaluate spontaneous nerve firing using in vivo electrophysiology.

Scientific Data

Changes in paw pressure (g) following Spinal Nerve Ligation in the SNL peripheral neuropathy model.

 

Paw Pressure_SNL Model_MD Biosciences
 
 
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Nerve injury datasheet (1)

 

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If you are ready to discuss how a partnership can fit into your development program, our scientists are eager to explore the possibilities with you. Like many other pharmaceutical and medical device developers, you can rely on predictive preclinical data.

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